Motagally et al from Queens University have published a literature on "Tumor necrosis factor {alpha} activates nuclear factor {kappa}B signalling to reduce N-type voltage-gated Ca2+ current in postganglionic sympathetic neurons" that was published in Journal of Physiology.
The article is based on characterizing the effect of overnight incubation in the inflammatory mediator TNFalpha (1 nM) on VGCC in dissociated neurons from mouse superior mesenteric ganglia (SMG).
Voltage-gated Ca2+ currents (ICa) were measured using the perforated patch clamp technique and the VGCC subtypes present in SMG neurons were estimated based on inhibition by selective VGCC blockers: omega-conotoxin GVIA (300 nM; N-type), nifedipine (10 muM; L-type), and omega-conotoxin MVIIC (300 nM; N, P/Q type).
Article Title: Tumor necrosis factor {alpha} activates nuclear factor {kappa}B signalling to reduce N-type voltage-gated Ca2+ current in postganglionic sympathetic neurons.
Source: Pubmed
PMID: 19403618
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